Inhibition of Polyuridylic Acid-Directed Protein Synthesis by Aurintricarboxylate in Extracts of Escherichia coli

Shigella toxin inhibition of binding and translation of polyuridylic acid by Escherichia coli ribosomes.

Shigella toxin inhibits polyuridylic acid-directed polymerization of phenylalanine in ribosome-enzyme systems obtained from Escherichia coli or from Shigella dysenteriae. The inhibition is the result of toxin acting on ribosomes to prevent polyuridylic acid attachment.

Deoxyribonucleic acid-directed in vitro synthesis of ilv-specific messenger ribonucleic acid by extracts of Escherichia coli K-12.

The synthesis of ilv-specific messenger ribonucleic acid (mRNA) by extracts of Escherichia coli K-12 has been demonstrated in a deoxyribonucleic acid (DNA)-dependent, coupled transcription-translation system. ilv-Specific mRNA was determined by hybridization either to double-stranded lambdacI857St68h80dilv DNA (lambdah80dilv DNA) immobilized on nitrocellulose filters or to its separate l and r ...

Transitory ambiguity in polyuridylic acid translation with Escherichia coli ribosomes.

Deoxyribonucleic acid repair in vitro by extracts of Escherichia coli.

Deoxyribonucleic acid (DNA) from bacteriophage T7 has been used to monitor the capacity of gently lysed extracts of Escherichia coli to perform repair resynthesis after ultraviolet (UV) irradiation. Purified DNA damaged by up to 100 J of UV radiation per m2 was treated with an endonuclease from Micrococcus luteus that introduces single-strand breaks in irradiated DNA. This DNA was then used as ...

Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.

l’rotein structure is under genetic control;’-3 yet the precise mechanism by which DNA$ influences the formation of specific amino acid sequences in proteins is unknown. Several years ago, it was discovered that infection of Escherichia coli with certain virulent bacteriophages induces the formation of an RNA fraction possessing both a high metabolic turnover rate and a base composition corresp...